Actual clinical­pathogenetic aspects of pathogenesis of adenomyosis and endometriomas for women different age

Abstract

DOI: 10.52705/2788-6190-2024-04.1-11
УДК 618.14-006.55-036-071.1

The objective: determination of role of allelic variants of genes of CYP19, GSTT1, GSTM1, р53 in pathogenesis and clinical motion of adenomyosis and ovarian endometriomas.
Materials and methods. 100 women with genital endometriosis and 50 women are inspected without this disease. Group 1 made 50 women from adenomyosis, middle age made them – in 44,9±0,7, group 2 made 50 women from ovarian endometriomas, middle age of 32,6±0,8. From them 10 patients with the relapse of ovarian endometriomas after the combined treatment. A diagnosis for all patients is verified intraoperatively and as a result of histological research. A control group was formed from 50 women at the inspection of which genital endometriosis was eliminated, without the clinical displays of violations ovarian-menstrual functions, in age from 17 to 35 years. For raising of diagnosis applied the general-clinical, ultrasonic, roentgenologic, endoscopic methods of inspection. During the executed work the special methods of researches are conducted: determination of level of SA-125 is in the whey of blood, molecular-genetic research of genes of GSTM1, GSTT1, R53, CYP19.
Results. As a result of the conducted analysis of polymorphic variants of gene of glutation S-transferase M1 it is not discovered statistically reliable differences in frequencies of deletions between the group of patients from adenomyosis and by a control group (52,0% and 42,0%, respectively). Frequency of a zero genotype in the group of patients practically did not differ from frequency of population. Frequency of homozygotes on zero allele of gene of glutation S-transferase M1 was some higher in the group of patients from adenomyosis as compared to the group of control (34,0% and 22,0%, accordingly). At the analysis of gene of glutation S-transferase M1 in the group of patients from ovarian endometriomas also it is not discovered statistically meaningful differences
with a control group in frequencies of zero genotypes (54,0% and 42,0%, respectively).
Conclusions. The results of the conducted researches testify that not on one of genes of GST not found out reliable differences between the groups of sick and healthy women, that some differentiates with information of literature. Presumably, it can be explained by that in an analysable group patients entered with severe endometriosis of the IV stage, while in previous researches conducted the analysis of endometriosis on the whole, without the account of the stage of disease. Possibly, at development of severe forms of disease there can be some other more serious breakages in a genome, that is scientific direction of our subsequent researches.